The demonstration in Edinburgh comes as Mr. Trump and first lady Melania Trump are spending the weekend out of the spotlight at his Turnberry resort in Scotland, ahead of his high-stakes summit on Monday with Russian President Vladimir Putin.
Saturday morning, Trump tweeted about the indictments.
While Trump took tea with Queen Elizabeth, a US federal grand jury charged 12 Russian intelligence officers with stealing data from the campaign of Democratic presidential candidate Hillary Clinton.
He told protesters: "Donald Trump is not welcome here".
"Where is the DNC Server, and why didn't the Federal Bureau of Investigation take possession of it?"
He also brought up his rejection of CNN's Jim Acosta Friday, during which he refused to take questions from the reporter at a British news conference.
Donald Trump keeps Queen waiting before Windsor Castle tea
Trump and the 92-year-old monarch shared a few interactions on Friday as they reviewed troops at the royal castle in Windsor . Donald Trump finally met Queen Elizabeth II after months of grumbling, and it went just about as well as you'd expect.
"I know loads of people who go there and loads of people who work there", Libby said. I got 306! They were sooooo wrong in their election coverage.
"The Stock Market hit 25,000 yesterday", wrote Trump. Trump often likes to remind people that he was elected president over Hillary Clinton.
For someone who claims extraordinary negotiation skills, Trump's forays into Scottish tourism and politics over the years have had mixed results.
Most of the controversy has centred on his other course, known as Trump International Golf Links. Most players at Turnberry, unless they have a note from their doctor, are required to walk the course, though they can hire caddies to wheel their bags of clubs.
He was also expected to see the burial grounds of his ancestors in Scotland, aides said. A line of police, some on horseback, separated the protesters from the golf course. "Trump is a liar!" as hotel guests played golf just 100 meters (yards) away.
He said his father could head to the north-east of Scotland during his visit, adding: "It's still to be decided".
At another point on the course, another group of protesters shouted "leave our country" from a nearby beach. We believe that this publicly-owned facility should not be used for Donald Trump's visit.
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I'm sure the players will be disappointed not to have reached the final, but there is so much to be hopeful about for the future. I don't think realistically any of us did", he said.
Across the park, past wobbling toddlers and wagging dogs, a similar story emerged as Scots, many of whom were new to political demonstrations, enjoyed a buoyant atmosphere with serious undertones, appreciating the opportunity to stand in solidarity with like-minded people across the United Kingdom and the world.
Ben Stewart, a spokesman for Greenpeace, said: the reaction from authorities was "pretty calm" when they informed them of the incoming stunt.
Protesters flew the same six-metre inflatable orange Trump baby balloon that was used near the British parliament in London on Friday.
When Trump arrived via motorcade on Friday evening, locals came out of their homes to wave and take photographs.
Police said the protester breached the no-fly zone. Meanwhile, Sen. John McCain, a leading Republican senator and Trump critic, has said Trump must hold Putin accountable or else he should not proceed with the meeting.
Leading Democratic senators asked Trump in a letter Saturday to scrap the summit "if you are not prepared to make Russia's attack on our election the top issue you will discuss".
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He never contacted authorities about the crash and refused to give a statement to police, once arrested, according to the charges. WABC reported the pair died from blunt force head trauma.