Scotland's parliament refused on Tuesday to give its consent for the European Union (Withdrawal) Bill, pushing Britain into constitutionally uncharted territory as London presses ahead with the bill regardless.
The vote came after months of wrangling over the bill's impact on devolved powers, and leaves the prime minister with the "high risk" decision between pressing ahead with the legislation despite its rejection by Holyrood, or making further concessions to the Scottish government to avoid a crisis, reports The Guardian.
Meanwhile, the UK Government has accused Scottish opponents of "nit-picking" and told its Scottish counterparts the "door is still open" for a deal to end the long-running Brexit powers dispute.
Scotland voted on independence in 2014, before Brexit.
The decision by the Scottish Parliament to refuse to give its consent to the Brexit Bill is a powerful, but largely symbolic, gesture.
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Ms. May had pledged to seek a Brexit that works for all the U.K.'s nations, but Scotland, which voted to remain in the European Union, is accusing her of a power grab.
Theresa May has the power to force the Bill through without getting the consent of Scotland.
The independence-minded Scottish National Party (SNP), which runs the minority government in Holyrood, Edinburgh, said it would be "outrageous" if the British government imposed the bill on Scotland.
It is the first time the devolved Parliament has withdrawn its stamp of approval for legislation coming from Westminster. "It's about a technical process of agreeing things that we've already agreed, and that's why I find it nearly incomprehensible that we've got into this debate around what's really a very, very technical issue".
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"It's profoundly regrettable that we don't have a deal in Scotland to allow us to move on".
Mrs May said the bill meant the devolved governments would still to be able to make all the decisions they can now make after Brexit.
'The blame for that lies entirely with the SNP.
Nicola Sturgeon's government in Edinburgh insists that Holyrood must explicity agree to any changes before they can take effect.
Peter Ross, Stranraer Liaison Group chairman, added: "We are delighted to be welcoming the Scottish Rural Parliament to Stranraer".
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Public talk of defying Brexit, though, dissipated after May's governing Conservative Party increased support in Scotland in last year's election, even as she lost her United Kingdom parliamentary majority.