A subsequent pathologist report found that Glushkov died by a "compression to the neck", London's Metropolitan Police said on Friday.
At this time, there is no link between Glushkov's death and the attempted murders of Skripal and his daughter, police said.
Mr Glushkov is the former deputy director of Russian state airline Aeroflot.
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It comes at a time when United Kingdom and Russian relations have sunk to fresh lows after the use of a nerve agent on United Kingdom soil against a former Russian spy who had previously passed information to Britain, which several world powers have blamed Russia for.
United Kingdom police say "there are no wider public health concerns" around the investigation into Glushkov's death.
Forensics investigators work at the home of Nikolai Glushkov in New Malden, on the outskirts of London, Britain, March 14, 2018.
He was found by police to have strangled himself five years ago at his home in Sunninghill, Berkshire, but later a coroner issued an open verdict on his death.
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They say counterterrorism detectives are leading the case "because of the associations Mr. Glushkov is believed to have had".
He served a five-year term for money laundering and fraud in Russian Federation, then fled the country after being handed a two-year suspended sentence for fraud in 2006.
Terror cops were treating Glushkov's death as unexplained but confirmed today a murder probe has been launched. In 2004, he was sentenced to three years and three months behind bars but was released in the courtroom having served the term in custody.
A murder investigation was launched Friday into yet another suspicious incident involving yet another Russian exile living in Britain - the latest episode coming just over a week after a former Kremlin spy and his daughter were poisoned. "It is unacceptable and we consider this situation as a failure of the UK's global liabilities under the Vienna convention of consular access", he said, adding that a relevant inquiry had been sent to the British authorities immediately after the news about businessman's death at the beginning of the week.
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Russian media said he was granted political asylum in Britain in 2010.